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PLANT HORMONES: METHODS AND PROTOCOLS, 3RD EDITION
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SYSNO ASEP 0487818 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana Author(s) Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Antoniadi, I. (SE)
Ljung, K. (SE)Number of authors 3 Source Title PLANT HORMONES: METHODS AND PROTOCOLS, 3RD EDITION. - Totowa : Humana Press, 2017 / KleineVehn J. ; Sauer M. - ISSN 1064-3745 - ISBN 978-1-4939-6467-3 Pages roč. 1497 (2017), s. 231-248 Series Methods in Molecular Biology Number of pages 18 s. Number of copy 2500 Number of pages 288 Publication form Print - P Language eng - English Country US - United States Keywords tandem mass-spectrometry ; solid-phase extraction ; gene-expression map ; plant-samples ; purification ; apex ; Cytokinins ; Fluorescence-activated cell sorting (FACS) ; Solid-phase extraction (SPE) ; Liquid chromatography-mass spectrometry (LC-MS) ; Roots ; Arabidopsis ; Protoplasts Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-34792S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000400364700020 DOI 10.1007/978-1-4939-6469-7_19 Annotation We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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