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Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode

SYSNO ASEP	0485557
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode
Author(s)	Špaček, Jan (BFU-R)_ORCID Daňhel, Aleš (BFU-R)_{RID, ORCID} Hasoň, Stanislav (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID}
Number of authors	4
Source Title	Electrochemistry Communications. - : Elsevier - ISSN 1388-2481 Roč. 82, SEP2017 (2017), s. 34-38
Number of pages	5 s.
Publication form	Print - P
Language	eng - English
Country	NL - Netherlands
Keywords	electrochemical oxidation ; nucleic-acids ; guanine ; reduction
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemistry and molecular biology
R&D Projects	GA16-01625S GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000408359600008
DOI	10.1016/j.elecom.2017.07.013
Annotation	An innovative approach to label-free voltammetric analysis of DNA at a pyrolytic graphite electrode (PGE) within a broad range of potentials (from2.0 to + 1.6 V) in an acetate buffer (pH 5) is presented. Using specifically designed DNA nonamers, we demonstrate not only anodic oxidation, but for the first time also cathodic reduction of nucleobases at the PGE. In addition, products of irreversible oxidation/reduction of the parent bases are shown to yield analytically useful, base-specific cathodic/anodic signals, making it possible to distinguish between the canonical bases (adenine, cytosine, guanine and thymine), uracil (U) and 5-methylcytosine (mC) in DNA. Furthermore, selective electrochemical switching off of the redox signals specific to certain nucleobases is presented as a way to resolve overlapping signals. Similarly, newly reported signals corresponding to electrochemically transformed bases can be switched on under specific conditions. This approach can be utilized for fast and facile simultaneous label-free analysis of bases in DNA, including mC and U, and to uncover overlapping signals. This significantly extends the possible applications of PGE in DNA research and (bio) sensor development.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2018

Number of the records: 1