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Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research
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SYSNO ASEP 0485263 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research Author(s) Kupcová Skalníková, Helena (UZFG-Y) RID, ORCID
Čížková, Jana (UZFG-Y)
Červenka, Jakub (UZFG-Y) ORCID
Vodička, Petr (UZFG-Y) ORCIDArticle number 2697 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 18, č. 12 (2017)Number of pages 32 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords cytokine ; cancer ; melanoma ; secretome ; proteomics Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemical research methods R&D Projects GA16-05534S GA ČR - Czech Science Foundation (CSF) LO1609 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 UT WOS 000418896700199 EID SCOPUS 85049870560 DOI 10.3390/ijms18122697 Annotation Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies. The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. Approaches covered span from mass spectrometry to immunoassays for single molecule detection (ELISA, western blot), multiplex assays (chemiluminescent, bead-based (Luminex) and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and emerging techniques for single cell secretomics). Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2018
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