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Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

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    SYSNO ASEP0485263
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleAdvances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research
    Author(s) Kupcová Skalníková, Helena (UZFG-Y) RID, ORCID
    Čížková, Jana (UZFG-Y)
    Červenka, Jakub (UZFG-Y) ORCID
    Vodička, Petr (UZFG-Y) ORCID
    Article number2697
    Source TitleInternational Journal of Molecular Sciences. - : MDPI
    Roč. 18, č. 12 (2017)
    Number of pages32 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordscytokine ; cancer ; melanoma ; secretome ; proteomics
    Subject RIVEB - Genetics ; Molecular Biology
    OECD categoryBiochemical research methods
    R&D ProjectsGA16-05534S GA ČR - Czech Science Foundation (CSF)
    LO1609 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUZFG-Y - RVO:67985904
    UT WOS000418896700199
    EID SCOPUS85049870560
    AnnotationMelanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies. The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. Approaches covered span from mass spectrometry to immunoassays for single molecule detection (ELISA, western blot), multiplex assays (chemiluminescent, bead-based (Luminex) and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and emerging techniques for single cell secretomics). Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication.
    WorkplaceInstitute of Animal Physiology and Genetics
    ContactJana Zásmětová,, Tel.: 315 639 554
    Year of Publishing2018
Number of the records: 1