Number of the records: 1
A New Approach to Heart Valve Tissue Engineering Based on Modifying Autologous Human Pericardium by 3D Cellular Mechanotransduction
- 1.
SYSNO ASEP 0484731 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A New Approach to Heart Valve Tissue Engineering Based on Modifying Autologous Human Pericardium by 3D Cellular Mechanotransduction Author(s) Straka, František (FGU-C)
Schorník, David (FGU-C)
Mašín, J. (CZ)
Filová, Elena (FGU-C) RID, ORCID
Miřejovský, T. (CZ)
Burdíková, Z. (US)
Švindrych, Z. (US)
Chlup, H. (CZ)
Horný, L. (CZ)
Veselý, J. (CZ)
Pirk, J. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDSource Title Journal of Biomaterials and Tissue Engineering - ISSN 2157-9083
Roč. 7, č. 7 (2017), s. 527-543Number of pages 17 s. Language eng - English Country US - United States Keywords autologous human pericardium ; pericardial interstitial cells ; heart valve ; 3D mechanotranduction ; bioreactor Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems R&D Projects NV15-29153A GA MZd - Ministry of Health (MZ) NT11270 GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000403660000003 EID SCOPUS 85019593898 DOI 10.1166/jbt.2017.1598 Annotation Objective: To perform a pilot study using a new method for preparing a three-cusp heart valve construct for potential use as a heart valve replacement based on modifying autologous human pericardium by 3D cellular mechanotransduction. Methods: Human pericardium samples were harvested during cardiac surgery and were cultured under dynamic conditions in the shape of the three cusp aortic heart valve for up to four weeks. After this time, the conditioned pericardial samples were compared with control unconditioned pericardial samples from the same patient and with a normal aortic heart valve obtained during heart transplantation. Results: Human pericardium consists of vimentin-positive pericardial interstitial cells which have similar properties to those of human valvular interstitial cells. These cells are able to respond to mechanical stresses through a process called mechanotransduction by proliferating and differentiating into an activated phenotype capable of producing new extracellular matrix. This was shown by a statistically significant increase in vimentin, alpha smooth muscle actin and Ki-67 positive cells after conditioning, and also by increased production of collagen I, elastin and glycosaminoglycans. The histological structure and the mechanical properties (TEV-secant elastic modulus E-s = 21.0 +/- 15.3 MPa) of the pericardial tissue engineered heart valve were positively changed and 3D dynamic conditioning was proven to be important for the activation of pericardial interstitial cells and for tissue remodeling. Conclusion: Autologous human pericardium may be a promising tissue from which to construct a living heart valve substitute with optimal mechanical and hemodynamic properties. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2018
Number of the records: 1