Number of the records: 1
Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization
- 1.
SYSNO ASEP 0481790 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization Author(s) Krajčovič, T. (SK)
Bučko, M. (SK)
Vikartovská, A. (SK)
Lacík, I. (SK)
Uhelská, L. (SK)
Chorvát, D. (SK)
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Gericke, M. (DE)
Heinze, T. (DE)
Gemeiner, P. (SK)Number of authors 11 Source Title Catalysts. - : MDPI
Roč. 7, č. 11 (2017), s. 353-364Number of pages 12 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords polyelectrolyte complex beads ; environmental scanning electron microscopy ; confocal laser scanning microscopy ; Baeyer-Villiger biooxidation ; cyclohexanone monoxygenase ; immobilization ; viable whole-cell biocatalyst Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation Institutional support UPT-D - RVO:68081731 UT WOS 000416780900042 EID SCOPUS 85035245420 DOI 10.3390/catal7110353 Annotation A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy, the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2018 Electronic address http://www.mdpi.com/2073-4344/7/11/353
Number of the records: 1