Number of the records: 1
Strong antimicrobial activity of xanthohumol and other derivatives from hops (Humulus lupulus L.) on gut anaerobic bacteria
- 1.
SYSNO ASEP 0481502 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Strong antimicrobial activity of xanthohumol and other derivatives from hops (Humulus lupulus L.) on gut anaerobic bacteria Author(s) Čermák, P. (CZ)
Olšovská, J. (CZ)
Mikyška, A. (CZ)
Dušek, M. (CZ)
Kadlečková, Z. (CZ)
Vaníček, J. (CZ)
Nyč, O. (CZ)
Sigler, Karel (MBU-M) RID
Bostíková, V. (CZ)
Bostík, P. (CZ)Source Title Acta Pathologica Microbiologica et Immunologica Scandinavica - ISSN 0903-4641
Roč. 125, č. 11 (2017), s. 1033-1038Number of pages 6 s. Language eng - English Country US - United States Keywords Xanthohumol ; gut ; anaerobic bacteria Subject RIV EE - Microbiology, Virology OECD category Microbiology Institutional support MBU-M - RVO:61388971 UT WOS 000413915600010 EID SCOPUS 85030637871 DOI 10.1111/apm.12747 Annotation Anaerobic bacteria, such as Bacteroides fragilis or Clostridium perfringens, are part of indigenous human flora. However, Clostridium difficile represents also an important causative agent of nosocomial infectious antibiotic-associated diarrhoea. Treatment of C.difficile infection is problematic, making it imperative to search for new compounds with antimicrobial properties. Hops (Humulus lupulus L.) contain substances with antibacterial properties. We tested antimicrobial activity of purified hop constituents humulone, lupulone and xanthohumol against anaerobic bacteria. The antimicrobial activity was established against B.fragilis, C.perfringens and C.difficile strains according to standard testing protocols (CLSI, EUCAST), and the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBC) were calculated. All C.difficile strains were toxigenic and clinically relevant, as they were isolated from patients with diarrhoea. Strongest antimicrobial effects were observed with xanthohumol showing MIC and MBC values of 15-107g/mL, which are close to those of conventional antibiotics in the strains of bacteria with increased resistance. Slightly higher MIC and MBC values were obtained with lupulone followed by higher values of humulone. Our study, thus, shows a potential of purified hop compounds, especially xanthohumol, as alternatives for treatment of infections caused by select anaerobic bacteria, namely nosocomial diarrhoea caused by resistant strains. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2018
Number of the records: 1