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Selective rab11 transport and the intrinsic regenerative ability of CNS axons

SYSNO ASEP	0481164
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Selective rab11 transport and the intrinsic regenerative ability of CNS axons
Author(s)	Koseki, H. (GB) Donegá, M. (GB) Lam, B.Y.H. (GB) Petrová, V. (GB) van Erp, S. (GB) Yeo, G.S.H. (GB) Kwok, Jessica (UEM-P)_{ORCID, RID} Ffrench-Constant, Ch. (GB) Eva, R. (GB) Fawcett, James (UEM-P)_ORCID
Source Title	eLife. - : eLife - ISSN 2050-084X Roč. 6, aug (2017), e26956
Number of pages	25 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	spinal-cord-injury ; chondroitin sulfate proteoglycans ; growth cone
Subject RIV	FH - Neurology
OECD category	Neurosciences (including psychophysiology
R&D Projects	EF15_003/0000419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEM-P - RVO:68378041
UT WOS	000411664100001
EID SCOPUS	85030710450
DOI	10.7554/eLife.26956
Annotation	Neurons lose intrinsic axon regenerative ability with maturation, but the mechanism remains unclear. Using an in-vitro laser axotomy model, we show a progressive decline in the ability of cut CNS axons to form a new growth cone and then elongate. Failure of regeneration was associated with increased retraction after axotomy. Transportation into axons becomes selective with maturation, we hypothesized that selective exclusion of molecules needed for growth may contribute to regeneration decline. With neuronal maturity rab11 vesicles (which carry many molecules involved in axon growth) became selectively targeted to the somatodendritic compartment and excluded from axons by predominant retrograde transport However, on overexpression rab11 was mistrafficked into proximal axons, and these axons showed less retraction and enhanced regeneration after axotomy. These results suggest that the decline of intrinsic axon regenerative ability is associated with selective exclusion of key molecules, and that manipulation of transport can enhance regeneration.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2018

Number of the records: 1