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Rapid identification of staphylococci by Raman spectroscopy
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SYSNO ASEP 0480832 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rapid identification of staphylococci by Raman spectroscopy Author(s) Rebrošová, K. (CZ)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Růžička, F. (CZ)
Bernatová, Silvie (UPT-D) RID, SAI
Holá, V. (CZ)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Sokolová, J. (CZ)
Petráš, P. (CZ)Number of authors 10 Article number 14846 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, NOV (2017), s. 1-8Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords coagulase-negative staphylococci ; Raman spectroscopy ; rapid identification Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GA15-20645S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000414233700066 EID SCOPUS 85032800977 DOI 10.1038/s41598-017-13940-w Annotation Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100 percent successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2018
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