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Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

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    SYSNO ASEP0480819
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleExtensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans
    Author(s) Sobotka, Roman (MBU-M) RID, ORCID
    Esson, H.J. (CZ)
    Koník, Peter (MBU-M)
    Trsková, Eliška (MBU-M)
    Moravcová, Lenka (MBU-M)
    Horák, A. (CZ)
    Dufková, P. (CZ)
    Oborník, Miroslav (MBU-M) RID
    Article number13214
    Source TitleScientific Reports - ISSN 2045-2322
    Roč. 7, OCT 16 (2017), s. 1-13
    Number of pages13 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsCYTOCHROME B(6)F COMPLEX ; WATER-WATER CYCLE ; LIGHT-HARVESTING ANTENNA
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsGBP501/12/G055 GA ČR - Czech Science Foundation (CSF)
    LO1416 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    EE2.3.30.0059 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportMBU-M - RVO:61388971
    UT WOS000413048000025
    EID SCOPUS85031790065
    DOI10.1038/s41598-017-13575-x
    AnnotationIn oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial and plastid photosystems, the core structures of PSI and PSII are conserved throughout photosynthetic eukaryotes. So far, the photosynthetic complexes have been characterised in only a small number of organisms. We performed in silico and biochemical studies to explore the organization and evolution of the photosynthetic apparatus in the chromerids Chromera velia and Vitrella brassicaformis, autotrophic relatives of apicomplexans. We catalogued the presence and location of genes coding for conserved subunits of the photosystems as well as cytochrome b(6)f and ATP synthase in chromerids and other phototrophs and performed a phylogenetic analysis. We then characterised the photosynthetic complexes of Chromera and Vitrella using 2D gels combined with mass-spectrometry and further analysed the purified Chromera PSI. Our data suggest that the photosynthetic apparatus of chromerids underwent unique structural changes. Both photosystems (as well as cytochrome b(6)f and ATP synthase) lost several canonical subunits, while PSI gained one superoxide dismutase (Vitrella) or two superoxide dismutases and several unknown proteins (Chromera) as new regular subunits. We discuss these results in light of the extraordinarily efficient photosynthetic processes described in Chromera.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2018
Number of the records: 1