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Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

SYSNO ASEP	0480819
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans
Author(s)	Sobotka, Roman (MBU-M)_{RID, ORCID} Esson, H.J. (CZ) Koník, Peter (MBU-M) Trsková, Eliška (MBU-M) Moravcová, Lenka (MBU-M) Horák, A. (CZ) Dufková, P. (CZ) Oborník, Miroslav (MBU-M)_RID
Article number	13214
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 7, OCT 16 (2017), s. 1-13
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	CYTOCHROME B(6)F COMPLEX ; WATER-WATER CYCLE ; LIGHT-HARVESTING ANTENNA
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	GBP501/12/G055 GA ČR - Czech Science Foundation (CSF)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EE2.3.30.0059 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000413048000025
EID SCOPUS	85031790065
DOI	10.1038/s41598-017-13575-x
Annotation	In oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial and plastid photosystems, the core structures of PSI and PSII are conserved throughout photosynthetic eukaryotes. So far, the photosynthetic complexes have been characterised in only a small number of organisms. We performed in silico and biochemical studies to explore the organization and evolution of the photosynthetic apparatus in the chromerids Chromera velia and Vitrella brassicaformis, autotrophic relatives of apicomplexans. We catalogued the presence and location of genes coding for conserved subunits of the photosystems as well as cytochrome b(6)f and ATP synthase in chromerids and other phototrophs and performed a phylogenetic analysis. We then characterised the photosynthetic complexes of Chromera and Vitrella using 2D gels combined with mass-spectrometry and further analysed the purified Chromera PSI. Our data suggest that the photosynthetic apparatus of chromerids underwent unique structural changes. Both photosystems (as well as cytochrome b(6)f and ATP synthase) lost several canonical subunits, while PSI gained one superoxide dismutase (Vitrella) or two superoxide dismutases and several unknown proteins (Chromera) as new regular subunits. We discuss these results in light of the extraordinarily efficient photosynthetic processes described in Chromera.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2018

Number of the records: 1