Number of the records: 1
Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization
- 1.
SYSNO ASEP 0480444 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization Author(s) Wang, G. (CN)
He, Q. (CN)
Macas, Jiří (BC-A) RID, ORCID, SAI
Novák, Petr (BC-A) RID, ORCID
Neumann, Pavel (BC-A) RID, ORCID
Meng, D. (CN)
Zhao, H. (CN)
Guo, N. (CN)
Han, S. (CN)
Zong, M. (CN)
Jin, W. (CN)
Liu, F. (CN)Number of authors 12 Source Title Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581
Roč. 152, č. 3 (2017), s. 158-165Number of pages 8 s. Publication form Print - P Language eng - English Country CH - Switzerland Keywords asymmetric somatic hybridization ; Fluorescence in situ hybridization ; Karyotype ; (Peri) centromere Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000412412600007 EID SCOPUS 85027723726 DOI 10.1159/000479179 Annotation Whole-genome shotgun reads were analyzed to determine the repeat sequence composition in the genome of black mustard, Brassica nigra (L.) Koch. The analysis showed that satellite DNA sequences are very abundant in the black mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers, their position and fluorescence intensity allowed for unambiguous identification of all 8 somatic metaphase chromosomes. A karyotype showing the location and fluorescence intensity of these tandem repeat sequences together with the position of rDNAs and centromeric retrotransposons of Brassica (CRB) was constructed. The establishment of the FISH-based karyotype in B. nigra provides valuable information that can be used in detailed analyses of B. nigra accessions and derived allopolyploid Brassica species containing the B genome. (C) 2017 S. Karger AG, Basel Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2018
Number of the records: 1