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The interplay of plasma treatment and gold coating and ultra-high molecular weight polyethylene: On the cytocompatibility

SYSNO ASEP	0480074
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The interplay of plasma treatment and gold coating and ultra-high molecular weight polyethylene: On the cytocompatibility
Author(s)	Novotná, Z. (CZ) Rimpelová, S. (CZ) Juřík, P. (CZ) Veselý, M. (CZ) Kolská, Z. (CZ) Hubáček, Tomáš (BC-A)_RID Ruml, T. (CZ) Švorčík, V. (CZ)
Number of authors	8
Source Title	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 71, FEB (2017), s. 125-131
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	UHMWPE ; plasma treatment ; gold sputtering ; atomic force microscopy
Subject RIV	JJ - Other Materials
OECD category	Materials engineering
R&D Projects	LM2015075 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344
UT WOS	000390967200016
EID SCOPUS	84992110508
DOI	10.1016/j.msec.2016.09.057
Annotation	We have investigated the application of Ar plasma for creation of nanostructured ultra high molecular weight polyethylene (PE) surface in order to enhance adhesion of mouse embryonic fibroblasts (L929). The aim of this study was to investigate the effect of the interface between plasma-treated and gold-coated PE on adhesion and spreading of cells. The surface properties of pristine samples and its modified counterparts were studied by different experimental techniques (gravimetry, goniometry and X-ray photoelectron spectroscopy (XPS), electrokinetic analysis), which were used for characterization of treated and sputtered layers, polarity and surface chemical structure, respectively. Further, atomic force microscopy (AFM) was employed to study the surface morphology and roughness. Biological responses of cells seeded on PE samples were evaluated in terms of cell adhesion, spreading, morphology and proliferation. Detailed cell morphology and intercellular connections were followed by scanning electron microscopy (SEM). As it was expected the thickness of a deposited gold film was an increasing function of the sputtering time. Despite the fact that plasma treatment proceeded in inert plasma, oxidized degradation products were formed on the PE surface which would contribute to increased hydrophilicity (wettability) of the plasma treated polymer. The XPS method showed a decrease in carbon concentration with increasing plasma treatment. Cell adhesion measured on the interface between plasma treated and gold coated PE was inversely proportional to the thickness of a gold layer on a sample.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2018

Number of the records: 1