Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization

Kaplan, Ondřej; Hierlemann, T.; Krajewski, S.; Kurz, J.; Nevoralová, Martina; Houska, Milan; Riedel, Tomáš; Riedelová, Zuzana; Zárubová, Jana; Wendel, H. P.; Brynda, Eduard

doi:https://dx.doi.org/10.1002/jbm.a.36152

Number of the records: 1

Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization

1.

SYSNO ASEP	0478912
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization
Author(s)	Kaplan, Ondřej (UMCH-V) Hierlemann, T. (DE) Krajewski, S. (DE) Kurz, J. (DE) Nevoralová, Martina (UMCH-V)_{RID, ORCID} Houska, Milan (UMCH-V)_RID Riedel, Tomáš (UMCH-V)_{RID, ORCID} Riedelová, Zuzana (UMCH-V)_ORCID Zárubová, Jana (FGU-C)_{RID, ORCID} Wendel, H. P. (DE) Brynda, Eduard (UMCH-V)_RID
Source Title	Journal of Biomedical Materials Research. Part A. - : Wiley - ISSN 1549-3296 Roč. 105, č. 11 (2017), s. 2995-3005
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	fibrin-heparin coating ; hemocompatibility ; endothelialization
Subject RIV	FA - Cardiovascular Diseases incl. Cardiotharic Surgery
OECD category	Cardiac and Cardiovascular systems
Subject RIV - cooperation	Institute of Physiology - Cardiovascular Diseases incl. Cardiotharic Surgery
R&D Projects	NV15-29153A GA MZd - Ministry of Health (MZ)
Institutional support	UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823
UT WOS	000411867800007
EID SCOPUS	85026837396
DOI	10.1002/jbm.a.36152
Annotation	Long-term performance of implanted cardiovascular grafts can be ensured if living endothelium overgrows their surface. Surface modifications to implants are therefore being sought that can encourage endothelialization while preventing thrombus formation until the natural endothelium is formed. In the present study, heparin was covalently attached to a fibrin mesh grown from a polyvinyl chloride (PVC) substrate surface by the catalytic action of surface immobilized thrombin on a fibrinogen solution. The coating prevented platelet activation, thrombin generation and clot formation, and reduced inflammatory reactions when exposed to fresh human whole blood circulating in a Chandler loop model. In addition, in vitro seeded human umbilical vein and human saphenous vein endothelial cells showed considerably enhanced attachment and proliferation on the coating.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2018

Number of the records: 1