Non-thermal air plasma promotes the healing of acute skin wounds in rats

Kubinová, Šárka; Závišková, Kristýna; Uherková, Lenka; Zablotskyy, Vitaliy A.; Churpita, Olexandr; Lunov, Oleg; Dejneka, Alexandr

doi:https://dx.doi.org/10.1038/srep45183

Number of the records: 1

Non-thermal air plasma promotes the healing of acute skin wounds in rats

1.

SYSNO ASEP	0478906
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Non-thermal air plasma promotes the healing of acute skin wounds in rats
Author(s)	Kubinová, Šárka (UEM-P)_{RID, ORCID} Závišková, Kristýna (UEM-P) Uherková, Lenka (UEM-P) Zablotskyy, Vitaliy A. (FZU-D)_RID Churpita, Olexandr (FZU-D)_{RID, ORCID} Lunov, Oleg (FZU-D)_ORCID Dejneka, Alexandr (FZU-D)_{RID, ORCID}
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 7, mar (2017), s. 45183
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	randomized controlled-trial ; atmospheric argon plasma ; nitric-oxide
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemical research methods
Subject RIV - cooperation	Institute of Physics - Biophysics
R&D Projects	LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	TA04010449 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Institutional support	UEM-P - RVO:68378041 ; FZU-D - RVO:68378271
UT WOS	000397295900001
EID SCOPUS	85016136253
DOI	10.1038/srep45183
Annotation	Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. We utilised a single jet NTP system generating atmospheric pressure air plasma, with ion volume density 5.10(17) m(-3) and gas temperature 30-35 degrees C. The skin wounds were exposed to three daily plasma treatments for 1 or 2 minutes and were evaluated 3, 7 and 14 days after the wounding by histological and gene expression analysis. NTP treatment significantly enhanced epithelization and wound contraction on day 7 when compared to the untreated wounds. Macrophage infiltration into the wound area was not affected by the NTP treatment. Gene expression analysis did not indicate an increased inflammatory reaction or a disruption of the wound healing proces, transient enhancement of inflammatory marker upregulation was found after NTP treatment on day 7. In summary, NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2018

Number of the records: 1