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RGB color coded images in scanning electron microscopy of biological surfaces

SYSNO ASEP	0478391
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	RGB color coded images in scanning electron microscopy of biological surfaces
Author(s)	Kofroňová, Olga (MBU-M)_{RID, ORCID} Benada, Oldřich (MBU-M)_{ORCID, RID}
Source Title	Acta virologica - ISSN 0001-723X Roč. 61, č. 3 (2017), s. 349-352
Number of pages	4 s.
Language	eng - English
Country	US - United States
Keywords	Biological surfaces ; Color images ; Scanning electron microscopy
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA16-20229S GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000411298700016
EID SCOPUS	85028583162
DOI	10.4149/av_2017_315
Annotation	We present here a methodological approach for the creation of color images in scanning electron microscopy by processing grayscale images taken simultaneously from at least three different detectors in a scanning electron microscope. The final color images are then produced by merging together those grayscale images in RGB color space. We show the images from non-conductive standard sample together with those obtained from real microbiological samples. The first one represents a microbial biofilm naturally grown on fiber glass filter. The other shows individual Bacillus subtilis cells from batch culture. All the image handling was done in open source image processing software ImageJ or GNU Image Manipulation Program (Gimp) or, alternatively, in proprietary AnalySis 3.2 Pro software processing suite.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2018

Number of the records: 1