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Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering
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SYSNO ASEP 0476688 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering Author(s) Šmít, Daniel (FGU-C)
Fouquet, C. (FR)
Pincet, F. (FR)
Zápotocký, Martin (FGU-C) RID, ORCID
Trembleau, A. (FR)Article number e19907 Source Title eLife. - : eLife - ISSN 2050-084X
Roč. 6, Apr 19 (2017)Number of pages 49 s. Language eng - English Country GB - United Kingdom Keywords biophysics ; cell adhesion ; coarsening ; developmental biology ; mathematical model ; mechanical tension ; axon guidance Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA14-16755S GA ČR - Czech Science Foundation (CSF) 7AMB12FR002 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000403786800001 EID SCOPUS 85021442510 DOI 10.7554/eLife.19907 Annotation While axon fasciculation plays a key role in the development of neural networks, very little is known about its dynamics and the underlying biophysical mechanisms. In a model system composed of neurons grown ex vivo from explants of embryonic mouse olfactory epithelia, we observed that axons dynamically interact with each other through their shafts, leading to zippering and unzippering behavior that regulates their fasciculation. Taking advantage of this new preparation suitable for studying such interactions, we carried out a detailed biophysical analysis of zippering, occurring either spontaneously or induced by micromanipulations and pharmacological treatments. We show that zippering arises from the competition of axon-axon adhesion and mechanical tension in the axons, and provide the first quantification of the force of axon-axon adhesion. Furthermore, we introduce a biophysical model of the zippering dynamics, and we quantitatively relate the individual zipper properties to global characteristics of the developing axon network. Our study uncovers a new role of mechanical tension in neural development: the regulation of axon fasciculation. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2018
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