Number of the records: 1

Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen

SYSNO ASEP	0476651
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
Author(s)	Landa, Přemysl (UEB-Q)_{RID, ORCID} Přerostová, Sylva (UEB-Q)_{RID, ORCID, SAI} Langhansová, Lenka (UEB-Q)_{RID, ORCID} Maršík, Petr (UEB-Q)_{RID, ORCID} Vaněk, Tomáš (UEB-Q)_{RID, ORCID}
Number of authors	5
Source Title	International Journal of Phytoremediation. - : Taylor & Francis - ISSN 1522-6514 Roč. 19, č. 8 (2017), s. 695-700
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	detoxification ; gene expression ; metabolism of xenobiotics ; microarrays ; phytoremediation
Subject RIV	CE - Biochemistry
OECD category	Plant sciences, botany
R&D Projects	GA14-22593S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000402041800001
EID SCOPUS	85019615193
DOI	10.1080/15226514.2016.1267697
Annotation	Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1