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Evaluation of drug uptake and deactivation in plant: Fate of albendazole in ribwort plantain (Plantago laceolata) cells and regenerants
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SYSNO ASEP 0476524 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evaluation of drug uptake and deactivation in plant: Fate of albendazole in ribwort plantain (Plantago laceolata) cells and regenerants Author(s) Stuchlíková Raisová, L. (CZ)
Podlipná, Radka (UEB-Q) RID, ORCID
Szotáková, B. (CZ)
Syslová, Eliška (UEB-Q)
Skálová, L. (CZ)Number of authors 5 Source Title Ecotoxicology and Enviromental Safety. - : Elsevier - ISSN 0147-6513
Roč. 141, JUL (2017), s. 37-42Number of pages 6 s. Language eng - English Country US - United States Keywords Anthelmintics ; Benzimidazoles ; Drug metabolism ; Drug uptake ; Phytoremediation Subject RIV CE - Biochemistry OECD category Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management R&D Projects GA15-05325S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000400880900006 EID SCOPUS 85015032189 DOI 10.1016/j.ecoenv.2017.03.014 Annotation Albendazole (ABZ) is a benzimidazole anthelmintic widely used especially in veterinary medicine. Along with other drugs, anthelmintics have become one of a new class of micro-pollutants that disturb the environment but the information about their fate in plants remains limited. The present study was designed to test the uptake and biotransformation of ABZ in the ribwort plantain (Plantago lancelota), a common meadow plant, which can come into contact with this anthelmintic through the excrements of treated animals in pastures. Two model systems were used and compared: cell suspensions and whole plant regenerants. In addition, time-dependent changes in occurrence of ABZ and its metabolites in roots, basal parts of the leaves and tops of the leaves were followed up. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 18 metabolites of ABZ formed in the ribwort. In both model systems, the same types of ABZ biotransformation reactions were found, but the spectrum and abundance of the ABZ metabolites detected in cell suspensions and regenerants differed significantly. Cell suspensions seem to be suitable only for qualitative estimations of drug biotransformation reactions while regenerants were shown to represent an adequate model for the qualitative as well as quantitative evaluation of drug uptake and metabolism in plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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