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Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

SYSNO ASEP	0475314
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut
Author(s)	Bodláková, K. (CZ) Jedlička, Pavel (UOCHB-X)_{ORCID, RID} Kodrík, Dalibor (BC-A)_{RID, ORCID}
Source Title	Insect Science. - : Wiley - ISSN 1672-9609 Roč. 24, č. 2 (2017), s. 259-269
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	AKH ; AKH receptor ; amylase ; enzyme ; gene expression ; midgut
Subject RIV	ED - Physiology
OECD category	Entomology
R&D Projects	GA14-07172S GA ČR - Czech Science Foundation (CSF)
Institutional support	UOCHB-X - RVO:61388963 ; BC-A - RVO:60077344
UT WOS	000398603200008
EID SCOPUS	84962641165
DOI	10.1111/1744-7917.12314
Annotation	This study examined the biochemical characteristics of alpha-amylase and hormonal (adipokinetic hormone: AKH) stimulation of alpha-amylase activity in the cockroach (Periplaneta americana) midgut. We applied two AKHs in vivo and in vitro, then measured resultant amylase activity and gene expression, as well as the expression of AKH receptor (AKHR). The results revealed that optimal amylase activity is characterized by the following: pH: 5.7, temperature: 38.4 degrees C, K-m (Michaelis-Menten constant): 2.54 mg starch/mL, and V-max (maximum reaction velocity): 0.185 mu mol maltose/mL/min. In vivo application of AKHs resulted in significant increase of amylase activity: by two-fold in the gastric caeca and 4-7 fold in the rest of the midgut. In vitro experiments supported results seen in vivo: a 24-h incubation with the hormones resulted in the increase of amylase activity by 1.4 times in the caeca and 4-9 times in the midgut. Further, gene expression analyses reveal that AKHR is expressed in both the caeca and the rest of the midgut, although expression levels in the former were 23 times higher than levels in the latter. A similar pattern was found for the amylase (AMY) gene. Hormonal treatment did not affect the expression of either gene. This study is the first to provide evidence indicating direct AKH stimulation of digestive enzyme activity in the insect midgut, supported by specific AKHR gene expression in this organ.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2018

Number of the records: 1