The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation

Sipošová, K.; Pospíšková, K.; Bednáriková, Z.; Šafařík, Ivo; Šafaříková, Miroslava; Kubovčíková, M.; Kopčanský, P.; Gázová, Z.

doi:https://dx.doi.org/10.1016/j.jmmm.2016.10.083

Number of the records: 1

The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation

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SYSNO ASEP	0474865
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation
Author(s)	Sipošová, K. (SK) Pospíšková, K. (CZ) Bednáriková, Z. (SK) Šafařík, Ivo (BC-A)_{RID, ORCID} Šafaříková, Miroslava (BC-A)_RID Kubovčíková, M. (SK) Kopčanský, P. (SK) Gázová, Z. (SK)
Source Title	Journal of Magnetism and Magnetic Materials. - : Elsevier - ISSN 0304-8853 Roč. 427, April (2017), s. 48-53
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	amyloid aggregation ; nanoparticles ; magnetic fluid ; dextran ; insulin
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
Institutional support	BC-A - RVO:60077344
UT WOS	000397199400010
EID SCOPUS	85006049767
DOI	10.1016/j.jmmm.2016.10.083
Annotation	Protein transformation from its soluble state into amyloid aggregates is associated with amyloid-related diseases. Amyloid deposits of insulin fibrils have been found in the sites of subcutaneous insulin application in patients with prolonged diabetes. Using atomic force microscopy and ThT fluorescence assay we have investigated the interference of insulin amyloid aggregation with superparamagnetic Fe3O4-based nanoparticles (SPIONs) coated with dextran (DEX), molecular mass of dextran was equal to 15-20, 40 or 70 kDa. The obtained data indicate that all three types of dextran coated nanoparticles (NP-FeDEXs) are able to inhibit insulin fibrillization and to destroy amyloid fibrils. The extent of anti-amyloid activities depends on the properties of NP-FeDEXs, mainly on the size of nanoparticles which is determined by molecular mass of dextran molecules. The most effective inhibiting activity was observed for the smallest nanoparticles coated with 1520 kDa dextran. Contrary, the highest destroying activity was observed for the largest NP-FeDEX (70 kDa dextran).
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2018

Number of the records: 1