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Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells

SYSNO ASEP	0474812
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells
Author(s)	Baldíková, E. (CZ) Pospíšková, K. (CZ) Ladakis, D. (GR) Kookos, I.K. (GR) Koutinas, A.A. (GR) Šafaříková, Miroslava (BC-A)_RID Šafařík, Ivo (BC-A)_{RID, ORCID}
Source Title	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 71, February (2017), s. 214-221
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	bacterial cellulose ; Komagataeibacter sucrofermentans ; copper phthalocyanine ; crystal violet ; yeast cells ; trypsin
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Institutional support	BC-A - RVO:60077344
UT WOS	000390967200027
EID SCOPUS	84991730101
DOI	10.1016/j.msec.2016.10.009
Annotation	Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal, the maximum adsorption capacity was 388 mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing, it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2018

Number of the records: 1