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Evaluation of the potential of chitosan/beta-1,3-glucan/hydroxyapatite material as a scaffold for living bone graft production in vitro by comparison of ADSC and BMDSC behaviour on its surface
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SYSNO ASEP 0474278 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evaluation of the potential of chitosan/beta-1,3-glucan/hydroxyapatite material as a scaffold for living bone graft production in vitro by comparison of ADSC and BMDSC behaviour on its surface Author(s) Przekora, A. (PL)
Vandrovcová, Marta (FGU-C) RID, ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Pajorová, Julia (FGU-C)
Molitor, M. (CZ)
Ginalska, G. (PL)
Bačáková, Lucie (FGU-C) RID, ORCIDArticle number 015030 Source Title Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 12, č. 1 (2017)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords runx2 ; adipose-derived stem cells ; bone marrow-derived stem cells ; osteogenic differentiation Subject RIV EI - Biotechnology ; Bionics OECD category Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials R&D Projects NV15-33018A GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000395985600005 EID SCOPUS 85016015656 DOI 10.1088/1748-605X/aa56f9 Annotation The opinion regarding the origin of adult stem cells that should be used for living bone construct generation is strongly divided in the scientific community. In this study, the potential of chitosan/beta-1,3glucan/hydroxyapatite (chit/glu/HA) material as a scaffold for bone regeneration applications was evaluated by behaviour comparison of adult stem cells derived from both origins-adipose derived mesenchymal stem cell (ADSC) tissue and bone marrow derived mesenchymal stem cells (BMDSCs). In the case of ADSC isolation, low and high negative pressures were applied during a liposuction procedure in order to determine if negative pressure settings may have an impact on subsequent cell behaviour in vitro. The obtained results demonstrated that the chit/glu/HA material is a promising candidate to be used for living bone graft production in vitro as both ADSCs and BMDSCs revealed a satisfactory proliferation and differentiation ability on its surface. Nevertheless, BMDSCs would be a better choice of adult stem cells since they were better spread, more strongly attached and showed a more superior proliferation and differentiation ability than ADSCs when cultured on the chit/glu/HA scaffold. However, if BMDSCs cannot be isolated, ADSCs may be used for bone construct production but lipoaspirate should be collected under low negative pressure (-200mm Hg), as high negative pressure (-700mm Hg) applied during liposuction surgery may retard subsequent ADSC proliferation and type I collagen production. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2018
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