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Catalyst-free „click” functionalization of polymer brushes preserves antifouling properties enabling detection in blood plasma
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SYSNO ASEP 0474248 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Catalyst-free „click” functionalization of polymer brushes preserves antifouling properties enabling detection in blood plasma Author(s) Parrillo, Viviana (UMCH-V)
de los Santos Pereira, Andres (UMCH-V) RID, ORCID
Riedel, Tomáš (UMCH-V) RID, ORCID
Rodriguez-Emmenegger, C. (DE)Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 971, 8 June (2017), s. 78-87Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords affinity optical biosensors ; blood plasma ; fouling Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GJ15-09368Y GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMCH-V - RVO:61389013 UT WOS 000400588500009 EID SCOPUS 85017459034 DOI 10.1016/j.aca.2017.03.007 Annotation Progress in biosensors for clinical detection critically relies on modifications of the transducer surface to prevent non-specific adsorption from matrix components (i.e. antifouling) while supporting biomolecular recognition elements to capture the analyte. Such combination of properties presents a significant challenge. Hierarchically structured polymer brushes comprising an antifouling polymer bottom block and a functionalizable top block are proposed as a promising strategy to achieve this goal. We employed the catalyst-free strain-promoted alkyne–azide cycloaddition (SPAAC) „click” reaction to biofunctionalize antifouling polymer brushes without impairing their resistance to fouling. The functionalization was performed on the side chains along the top polymer block or only on the end-groups of the polymer brush. The immobilized amounts of bioreceptors (streptavidin followed by biotin-conjugated proteins) and the resistance to fouling from blood plasma of the surfaces obtained were evaluated via surface plasmon resonance. The end group functionalization approach resulted in very low immobilization of bioreceptor. On the other hand, the side group modification of a top polymer block led to immobilization of 83% of a monolayer of streptavidin. Following binding of a biotin-conjugated antibody (66 ng cm-2) the functionalized layer was able to reduce the fouling from undiluted human blood plasma by 89% in comparison with bare gold. Finally, the functionalized hierarchical polymer brushes were applied to the label-free detection of a model analyte in diluted human blood plasma, highlighting the potential for translation to medical applications. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2018
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