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Basal and Activated Calcium Sensitization Mediated by RhoA/Rho Kinase Pathway in Rats with Genetic and Salt Hypertension

SYSNO ASEP	0473932
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Basal and Activated Calcium Sensitization Mediated by RhoA/Rho Kinase Pathway in Rats with Genetic and Salt Hypertension
Author(s)	Behuliak, Michal (FGU-C)_{RID, ORCID} Bencze, Michal (FGU-C)_{RID, ORCID, SAI} Vaněčková, Ivana (FGU-C)_{RID, ORCID} Kuneš, Jaroslav (FGU-C)_{RID, ORCID} Zicha, Josef (FGU-C)_{RID, ORCID, SAI}
Article number	8029728
Source Title	BioMed Research International. - : Hindawi - ISSN 2314-6133 Roč. 2017, January (2017)
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	calcium sensitization ; RhoA/Rho kinase ; fasudil ; calcium influx ; nifedipine ; BAY K8644
Subject RIV	FA - Cardiovascular Diseases incl. Cardiotharic Surgery
OECD category	Cardiac and Cardiovascular systems
R&D Projects	GP14-16225P GA ČR - Czech Science Foundation (CSF)
	NV15-25396A GA MZd - Ministry of Health (MZ)
Institutional support	FGU-C - RVO:67985823
UT WOS	000394007500001
EID SCOPUS	85011931119
DOI	10.1155/2017/8029728
Annotation	Calcium sensitization mediated by RhoA/Rho kinase pathway can be evaluated either in the absence (basal calcium sensitization) or in the presence of endogenous vasoconstrictor systems (activated calcium sensitization). Our aim was to compare basal and activated calcium sensitization in three forms of experimental hypertension with increased sympathetic tone and enhanced calcium entry—spontaneously hypertensive rats (SHR), heterozygous Ren-2 transgenic rats (TGR), and salt hypertensive Dahl rats. Activated calcium sensitization was determined as blood pressure reduction induced by acute administration of Rho kinase inhibitor fasudil in conscious rats with intact sympathetic nervous system (SNS) and renin-angiotensin system (RAS). Basal calcium sensitization was studied as fasudil-dependent difference in blood pressure response to calcium channel opener BAY K8644 in rats subjected to RAS and SNS blockade. Calcium sensitization was also estimated from reduced development of isolated artery contraction by Rho kinase inhibitor Y-27632. Activated calcium sensitization was enhanced in all three hypertensive models (due to the hyperactivity of vasoconstrictor systems). In contrast, basal calcium sensitization was reduced in SHR and TGR relative to their controls, whereas it was augmented in salt-sensitive Dahl rats relative to their salt-resistant controls. Similar differences in calcium sensitization were seen in femoral arteries of SHR and Dahl rats.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2018

Number of the records: 1