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The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite
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SYSNO ASEP 0473017 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite Author(s) Yurchenko, T. (CZ)
Ševčíková, T. (CZ)
Strnad, Hynek (UMG-J) RID
Butenko, A. (CZ)
Eliáš, M. (CZ)Number of authors 5 Article number 160249 Source Title Open Biology. - : Royal Society Publishing
Roč. 6, č. 11 (2016)Number of pages 22 Language eng - English Country GB - United Kingdom Keywords Eustigmatophyceae ; horizontal gene transfer ; plastid genome ; secondary metabolism ; sugar phosphate cyclase superfamily ; UbiA superfamily Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA13-33039S GA ČR - Czech Science Foundation (CSF) ED2.1.00/19.0388 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1208 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000390348200013 DOI 10.1098/rsob.160249 Annotation Acquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case of HGT revealed by sequencing the plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP Q 202. These plastomes proved to harbour a unique cluster of six genes, most probably acquired from a bacterium of the phylum Bacteroidetes, with homologues in various bacteria, typically organized in a conserved uncharacterized putative operon. Sequence analyses of the six proteins encoded by the operon yielded the following annotation for them: (i) a novel family without discernible homologues; (ii) a new family within the superfamily of metallo-dependent hydrolases; (iii) a novel subgroup of the UbiA superfamily of prenyl transferases; (iv) a new clade within the sugar phosphate cyclase superfamily; (v) a new family within the xylose isomerase-like superfamily; and (vi) a hydrolase for a phosphate moietycontaining substrate. We suggest that the operon encodes enzymes of a pathway synthesizing an isoprenoid-cyclitol-derived compound, possibly an antimicrobial or other protective substance. To the best of our knowledge, this is the first report of an expansion of the metabolic capacity of a plastid mediated by HGT into the plastid genome. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2017
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