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p53 binds human telomeric G-quadruplex in vitro
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SYSNO ASEP 0471957 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title p53 binds human telomeric G-quadruplex in vitro Author(s) Adámik, Matěj (BFU-R) ORCID
Kejnovská, Iva (BFU-R) RID, ORCID
Bažantová, Pavla (BFU-R)
Petr, Marek (BFU-R) ORCID
Renčiuk, Daniel (BFU-R) RID, ORCID
Vorlíčková, Michaela (BFU-R) RID, ORCID
Brázdová, Marie (BFU-R) RID, ORCIDNumber of authors 7 Source Title Biochimie. - : Elsevier - ISSN 0300-9084
Roč. 128, SEPT2016 (2016), s. 83-91Number of pages 9 s. Publication form Print - P Language eng - English Country FR - France Keywords crystal-structure ; human-chromosomes ; supercoiled dna Subject RIV BO - Biophysics R&D Projects GA13-36108S GA ČR - Czech Science Foundation (CSF) GP14-33947P GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000385327700009 DOI 10.1016/j.biochi.2016.07.004 Annotation The tumor suppressor protein p53 is a key factor in genome stability and one of the most studied of DNA binding proteins. This is the first study on the interaction of wild-type p53 with guanine quadruplexes formed by the human telomere sequence. Using electromobility shift assay and ELISA, we show that p53 binding to telomeric G-quadruplexes increases with the number of telomeric repeats. Further, p53 strongly favors G-quadruplexes folded in potassium over those formed in sodium, thus indicating the telomeric G-quadruplex conformational selectivity of p53. The presence of the quadruplex-stabilizing ligand, N-methyl mesoporphyrin IX (NMM), increases p53 recognition of G-quadruplexes in potassium. Using deletion mutants and selective p53 core domain oxidation, both p53 DNA binding domains are shown to be crucial for telomeric G-quadruplex recognition. (C) 2016 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2017
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