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Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer
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SYSNO ASEP 0471472 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer Author(s) Žáček, Petr (UOCHB-X) RID
Bukowski, M. (US)
Rosenberger, T. A. (US)
Picklo, M. (US)Source Title Journal of Lipid Research. - : Elsevier - ISSN 0022-2275
Roč. 57, č. 12 (2016), s. 2225-2234Number of pages 10 s. Language eng - English Country US - United States Keywords shotgun lipidomics ; triple quadrupole/ion-trap ; human blood plasma ; phosphatidylcholines Subject RIV CB - Analytical Chemistry, Separation Institutional support UOCHB-X - RVO:61388963 UT WOS 000389473000014 EID SCOPUS 85002583278 DOI 10.1194/jlr.D070656 Annotation Phosphatidylcholine (PC) species in human plasma are used as biomarkers of disease. PC biomarkers are often limited by the inability to separate isobaric PCs. In this work, we developed a targeted shotgun approach for analysis of isobaric and isomeric PCs. This approach is comprised of two MS methods: a precursor ion scanning (PIS) of mass m/z 184 in positive mode (PIS m/z +184) and MS3 fragmentation in negative mode, both performed on the same instrument, a hybrid triple quadrupole ion-trap mass spectrometer. The MS3 experiment identified the FA composition and the relative abundance of isobaric and sn-1, sn-2 positional isomeric PC species, which were subsequently combined with absolute quantitative data obtained by PIS m/z +184 scan. This approach was applied to the analysis of a National Institute of Standards and Technology human blood plasma standard reference material (SRM 1950). We quantified more than 70 PCs and confirmed that a majority are present in isobaric and isomeric mixtures. The FA content determined by this method was comparable to that obtained using GC with flame ionization detection, supporting the quantitative nature of this MS method. This methodology will provide more in-depth biomarker information for clinical and mechanistic studies. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2017 Electronic address http://www.jlr.org/content/57/12/2225.full
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