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Dipolar Relaxation Dynamics at the Active Site of an ATPase Regulated by Membrane Lateral Pressure

SYSNO ASEP	0471291
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Dipolar Relaxation Dynamics at the Active Site of an ATPase Regulated by Membrane Lateral Pressure
Author(s)	Fischermeier, E. (DE) Pospíšil, Petr (UFCH-W) Sayed, A. (DE) Hof, Martin (UFCH-W)_{RID, ORCID} Solioz, M. (CH) Fahmy, K. (DE)
Number of authors	6
Source Title	Angewandte Chemie - International Edition. - : Wiley - ISSN 1433-7851 Roč. 56, č. 5 (2017), s. 1269-1272
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	fluorescence ; ion pump ; membrane proteins ; nanodiscs ; time-resolved emission
Subject RIV	CF - Physical ; Theoretical Chemistry
OECD category	Physical chemistry
R&D Projects	GBP208/12/G016 GA ČR - Czech Science Foundation (CSF)
Institutional support	UFCH-W - RVO:61388955
UT WOS	000394997700014
EID SCOPUS	85007324122
DOI	10.1002/anie.201611582
Annotation	The active transport of ions across biological membranes requires their hydration shell to interact with the interior of membrane proteins. However, the influence of the external lipid phase on internal dielectric dynamics is hard to access by experiment. Using the octahelical transmembrane architecture of the copper-transporting P1B-type ATPase from Legionella pneumophila as a model structure, we have established the site-specific labeling of internal cysteines with a polarity-sensitive fluorophore. This enabled dipolar relaxation studies in a solubilized form of the protein and in its lipid-embedded state in nanodiscs. Time-dependent fluorescence shifts revealed the site-specific hydration and dipole mobility around the conserved ion-binding motif. The spatial distribution of both features is shaped significantly and independently of each other by membrane lateral pressure.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2018

Number of the records: 1