Number of the records: 1

BFPTool: a software tool for analysis of Biomembrane Force Probe experiments

SYSNO ASEP	0471014
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	BFPTool: a software tool for analysis of Biomembrane Force Probe experiments
Author(s)	Šmít, Daniel (FGU-C) Fouquet, C. (FR) Doulazmi, M. (FR) Pincet, F. (FR) Trembleau, A. (FR) Zápotocký, Martin (FGU-C)_{RID, ORCID}
Article number	2
Source Title	BMC Biophysics Roč. 10, Feb (2017)
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Biomembrane Force Probe ; motion tracking ; image analysis ; force spectroscopy ; cell mechanics
Subject RIV	BO - Biophysics
OECD category	Biophysics
R&D Projects	GA14-16755S GA ČR - Czech Science Foundation (CSF)
	7AMB12FR002 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FGU-C - RVO:67985823
UT WOS	000397045500001
EID SCOPUS	85012914208
DOI	10.1186/s13628-016-0033-2
Annotation	The Biomembrane Force Probe is an approachable experimental technique commonly used for single-molecule force spectroscopy and experiments on biological interfaces. The technique operates in the range of forces from 0.1 pN to 1000 pN. Experiments are typically repeated many times, conditions are often not optimal, the captured video can be unstable and lose focus; this makes efficient analysis challenging, while out-of-the-box non-proprietary solutions are not freely available.This dedicated tool was developed to integrate and simplify the image processing and analysis of videomicroscopy recordings from BFP experiments. A novel processing feature, allowing the tracking of the pipette, was incorporated to address a limitation of preceding methods. Emphasis was placed on versatility and comprehensible user interface implemented in a graphical form.An integrated analytical tool was implemented to provide a faster, simpler and more convenient way to process and analyse BFP experiments.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2017

Number of the records: 1