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Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
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SYSNO ASEP 0470315 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization Author(s) Doubková, Martina (FGU-C) ORCID, RID
Bačáková, Lucie (FGU-C) RID, ORCID
Pařízek, Martin (FGU-C) RID
Vandrovcová, Marta (FGU-C) RID, ORCID
Musílková, Jana (FGU-C) RID, ORCID
Lisá, Věra (FGU-C)
Gabor, R. (CZ)
Marvan, J. (CZ)Source Title NANOCON 2015: 7th International Conference on Nanomaterials - Research and Application, Conference Proceedings. - Ostrava : TANGER, spol. s r.o., 2015 - ISBN 978-80-87294-63-5 Pages s. 460-465 Number of pages 6 s. Publication form Print - P Action NANOCON 2015. International Conference /7./ Event date 14.10.2015 - 16.10.2015 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords Ti6-Al4-V ; SAOS-2 cells ; anodization ; bone implants ; collagen I ; mineralization Subject RIV EI - Biotechnology ; Bionics R&D Projects TA04011214 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) NV15-32497A GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000374708800081 EID SCOPUS 84962822286 Annotation Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2017
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