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Characterization of WEE1 regulation mutant of the green alga Chlamydomonas reinhardtii in the response to DNA damage
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SYSNO ASEP 0469989 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Characterization of WEE1 regulation mutant of the green alga Chlamydomonas reinhardtii in the response to DNA damage Author(s) Hlavová, Monika (MBU-M)
Čížková, Mária (MBU-M) ORCID
Turóczy, Zoltán (MBU-M)
Bišová, Kateřina (MBU-M) RIDSource Title Book of Abstract of the 17th International Conference on the Cell and Molecular Biology of Chlamydomonas. - Kyoto, 2016 Action 17th International Conference on Cell and Molecular Biology of Chlamydomonas Event date 26.06.2016 - 01.07.2016 VEvent location Kyoto Country JP - Japan Event type WRD Language eng - English Country JP - Japan Subject RIV EA - Cell Biology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 Annotation Eukaryotic cell cycle progression can be affected by various mechanisms. One of them, DNA damage checkpoint, arrests the cell cycle after incorrect DNA replication or DNA damage. To block cell cycle, DNA damage checkpoint may exploit Wee1 kinase. Our preliminary results suggested Wee1 is involved both in the regulation of mitosis and in the response to DNA damage but the surrounding gene/protein network remains unknown. We screened 20,000 insertional mutants to isolate those with altered expression of CrWEE1 upon
DNA damage and we were able to recovered 14 of them, called wer (WEE1 regulation) mutants. One of the most interesting mutants, wer12, showed very high sensitivity to zeocin. On the first sight, we did not detect any cell cycle related phenotype. The division was, however, aberrant with irregular numbers of daughter cells of different cell size. The most striking phenotype was the extremely small daughter cells resembling mat3-4 phenotype. The wer12 cell size phenotype was stable both in untreated and in zeocin-treated conditions suggesting it is unrelated to the observed DNA damage response phenotype.
Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
Number of the records: 1