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The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow
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SYSNO ASEP 0469068 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow Author(s) Szöke, K. (NO)
Daňková, Jana (UEM-P)
Buzgo, Matej (UEM-P)
Amler, Evžen (UEM-P) RID
Brinchmann, J.E. (NO)
Ostrup, E. (NO)Source Title Process Biochemistry. - : Elsevier - ISSN 1359-5113
Roč. 59, B (2017), s. 321-328Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords mesenchymal stem cells ; polycaprolactone ; osteogenic differentiation Subject RIV FP - Other Medical Disciplines OECD category Cell biology Institutional support UEM-P - RVO:68378041 UT WOS 000412376700023 EID SCOPUS 85001969692 DOI 10.1016/j.procbio.2016.10.011 Annotation The two mesenchymal stem cell (MSC) populations that have gained most attention in relation to bone tissue engineering are adipose tissue (AT) MSCs and bone marrow (BM) MSCs. The purpose of this study was to investigate the ability of human BM-MSCs and AT-MSCs to survive, proliferate and deposit collagen type 1 when cultured on polycaprolactone nanofiber scaffolds and to ascertain the effect of medium composition on collagen type 1 formation and expression of osteogenic genes. The cells were seeded on polycaprolactone nanofiber scaffolds and cultured in three different types of media that differed by the presence of ascorbic acid,beta-glycerophosphate and dexamethasone, that are typical components used for osteogenic differentiation of MSCs in vitro.
In summary, AT-MSCs were proliferating significantly faster than BM-MSCs. AT-MSCs also showed better ability to deposit collagen type 1 and had a higher expression of early osteogenic markers, whereas BM-MSCs had higher expression of late osteogenic markers. This suggests that MSCs from diverse sources have different attributes and with respect to osteogenic differentiation, AT-MSCs are more immature compared to BM-MSCs. Collagen formation was depending on medium composition and the organization of collagen type I appeared to be influenced by the presence of dexamethasone.Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2018
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