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Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles

SYSNO ASEP	0469034
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles
Author(s)	Dvořák, Jan (BC-A) Fajtová, P. (CZ) Ulrychová, L. (CZ) Leontovyc, A. (CZ) Rojo-Arreola, L. (US) Suzuki, B.M. (US) Horn, M. (CZ) Mareš, M. (CZ) Craik, C. S. (US) Caffrey, C. R. (US) O'Donoghue, A.J. (US)
Number of authors	11
Source Title	Biochimie. - : Elsevier - ISSN 0300-9084 Roč. 122, MAR (2016), s. 99-109
Number of pages	11 s.
Publication form	Print - P
Language	eng - English
Country	FR - France
Keywords	parasite ; fluke ; secretion ; excretion ; protease ; inhibitor
Subject RIV	EB - Genetics ; Molecular Biology
Institutional support	BC-A - RVO:60077344
UT WOS	000370910700010
EID SCOPUS	84957794203
DOI	10.1016/j.biochi.2015.09.025
Annotation	Schistosomiasis is one of a number of chronic helminth diseases of poverty that severely impact personal and societal well-being and productivity. Peptidases (proteases) are vital to successful parasitism, and Can modulate host physiology and immunology. Interference of peptidase action by specific drugs or vaccines can be therapeutically beneficial. To date, research on peptidases in the schistosome parasite has focused on either the functional characterization of individual peptidases or their annotation as part of global genome or transcriptome studies. We were interested in functionally characterizing the complexity of peptidase activity operating at the host parasite interface, therefore the excretory secretory products of key developmental stages of Schistosoma mansoni that parasitize the human were examined. Using class specific peptidase inhibitors in combination with a multiplex substrate profiling assay, a number of unique activities derived from endo- and exo-peptidases were revealed in the excretory-secretory products of schistosomula (larval migratory worms), adults and eggs. The data highlight the complexity of the functional degradome for each developmental stage of this parasite and facilitate further enquiry to establish peptidase identity, physiological and immunological function, and utility as drug or vaccine candidates. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2017

Number of the records: 1