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Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)

SYSNO ASEP	0468874
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
Author(s)	Jerez, C.G. (ES) Malapascua, José R. (MBU-M) Sergejevová, Magda (MBU-M) Figueroa, Felix L. (ES) Masojídek, Jiří (MBU-M)_{RID, ORCID}
Source Title	Marine Biotechnology - ISSN 1436-2228 Roč. 18, č. 1 (2016), s. 24-36
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	Microalgae ; Chlorella ; Lipid
Subject RIV	EE - Microbiology, Virology
R&D Projects	ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EE2.3.30.0059 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EE2.3.20.0203 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000378136600003
EID SCOPUS	84953354282
DOI	10.1007/s10126-015-9664-6
Annotation	The effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1