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Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)

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    SYSNO ASEP0468874
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleEffect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
    Author(s) Jerez, C.G. (ES)
    Malapascua, José R. (MBU-M)
    Sergejevová, Magda (MBU-M)
    Figueroa, Felix L. (ES)
    Masojídek, Jiří (MBU-M) RID, ORCID
    Source TitleMarine Biotechnology - ISSN 1436-2228
    Roč. 18, č. 1 (2016), s. 24-36
    Number of pages13 s.
    Languageeng - English
    CountryUS - United States
    KeywordsMicroalgae ; Chlorella ; Lipid
    Subject RIVEE - Microbiology, Virology
    R&D ProjectsED2.1.00/03.0110 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    EE2.3.30.0059 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    EE2.3.20.0203 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportMBU-M - RVO:61388971
    UT WOS000378136600003
    EID SCOPUS84953354282
    DOI10.1007/s10126-015-9664-6
    AnnotationThe effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2017
Number of the records: 1