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MYD88 and functionally related genes are associated with multiple infections in a model population of Kenyan village dogs
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SYSNO ASEP 0468783 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title MYD88 and functionally related genes are associated with multiple infections in a model population of Kenyan village dogs Author(s) Nečesanková, M. (CZ)
Vychodilová, L. (CZ)
Albrechtová, K. (CZ)
Kennedy, L. J. (GB)
Hlaváč, J. (CZ)
Sedlák, K. (CZ)
Modrý, David (BC-A) RID, ORCID
Jánová, E. (CZ)
Vyskočil, M. (CZ)
Horin, P. (CZ)Number of authors 10 Source Title Molecular Biology Reports. - : Springer - ISSN 0301-4851
Roč. 43, č. 12 (2016), s. 1451-1463Number of pages 13 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Kenyan village dogs ; immunity-related genes ; associations ; infectious diseases Subject RIV EB - Genetics ; Molecular Biology Institutional support BC-A - RVO:60077344 UT WOS 000387671000012 EID SCOPUS 84988606149 DOI 10.1007/s11033-016-4078-8 Annotation The purpose of this study was to seek associations between immunity-related molecular markers and endemic infections in a model population of African village dogs from Northern Kenya with no veterinary care and no selective breeding. A population of village dogs from Northern Kenya composed of three sub-populations from three different areas (84, 50 and 55 dogs) was studied. Canine distemper virus (CDV), Hepatozoon canis, Microfilariae (Acantocheilonema dracunculoides, Acantocheilonema reconditum) and Neospora caninum were the pathogens studied. The presence of antibodies (CDV, Neospora), light microscopy (Hepatozoon) and diagnostic PCR (Microfilariae) were the methods used for diagnosing infection. Genes involved in innate immune mechanisms, NOS3, IL6, TLR1, TLR2, TLR4, TLR7, TLR9, LY96, MYD88, and three major histocompatibility genes class II genes were selected as candidates. Single nucleotide polymorphism (SNP) markers were detected by Sanger sequencing, next generation sequencing and PCR-RFLP. The FisherA ' s exact test for additive and non-additive models was used for association analyses. Three SNPs within the MYD88 gene and one TLR4 SNP marker were associated with more than one infection. Combined genotypes and further markers identified by next generation sequencing confirmed associations observed for individual genes. The genes associated with infection and their combinations in specific genotypes match well our knowledge on their biological role and on the role of the relevant biological pathways, respectively. Associations with multiple infections observed between the MYD88 and TLR4 genes suggest their involvement in the mechanisms of anti-infectious defenses in dogs. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
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