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Redox regulation of ATP sulfurylase in microalgae
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SYSNO ASEP 0468470 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Redox regulation of ATP sulfurylase in microalgae Author(s) Prioretti, L. (IT)
Lebrun, R. (FR)
Gontero, B. (FR)
Giordano, Mario (MBU-M) ORCIDSource Title Biochemical and Biophysical Research Communications. - : Elsevier - ISSN 0006-291X
Roč. 478, č. 4 (2016), s. 1555-1562Number of pages 9 s. Language eng - English Country US - United States Keywords ATP sulfurylase ; cysteine ; Sulfur metabolism Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000384390200011 EID SCOPUS 84992107986 DOI 10.1016/j.bbrc.2016.08.151 Annotation ATP sulfurylase (ATPS) catalyzes the first step of sulfur assimilation in photosynthetic organisms. An ATPS type A is mostly present in freshwater cyanobacteria, with four conserved cysteine residues. Oceanic cyanobacteria and most eukaryotic algae instead, possess an ATPS-B containing seven to ten cysteines; five of them are conserved, but only one in the same position as ATPS-A. We investigated the role of cysteines on the regulation of the different algal enzymes. We found that the activity of ATPS-B from four different microorganisms was enhanced when reduced and decreased when oxidized. The LC-MS/MS analysis of the ATPS-B from the marine diatom Thalassiosira pseudonana showed that the residue Cys-247 was presumably involved in the redox regulation. The absence of this residue in the ATPS-A of the freshwater cyanobacterium Synechocystis sp. instead, was consistent with its lack of regulation. Some other conserved cysteine residues in the ATPS from T. pseduonana and not in Synechocystis sp. were accessible to redox agents and possibly play a role in the enzyme regulation. Furthermore, the fact that oceanic cyanobacteria have ATPS-B structurally and functionally closer to that from most of eukaryotic algae than to the ATPS-A from other cyanobacteria suggests that life in the sea or freshwater may have driven the evolution of ATPS. ( Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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