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Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii)
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SYSNO ASEP 0468300 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii) Author(s) Jirků-Pomajbíková, Kateřina (BC-A) ORCID, RID
Čepička, I. (CZ)
Kalousová, B. (CZ)
Jirků, Milan (BC-A) RID
Stewart, F. (GB)
Levecke, B. (BE)
Modrý, David (BC-A) RID, ORCID
Piel, A. K. (GB)
Petrželková, Klára Judita (BC-A) RIDNumber of authors 9 Source Title Parasitology. - : Cambridge University Press - ISSN 0031-1820
Roč. 143, č. 6 (2016), s. 741-748Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Entamoeba ; molecular diversity ; great apes ; chimpanzee ; savannah Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA206/09/0927 GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000376783000008 EID SCOPUS 84960108987 DOI 10.1017/S0031182016000263 Annotation To address the molecular diversity and occurrence of pathogenic species of the genus Entamoeba spp. in wild non-human primates (NHP) we conducted molecular-phylogenetic analyses on Entamoeba from wild chimpanzees living in the Issa Valley, Tanzania. We compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (PCR)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of Entamoeba spp. We identified Entamoeba spp. in 72 chimpanzee fecal samples (79%) subjected to species-specific PCRs for six Entamoeba species/groups (Entamoeba histolytica, Entamoeba nuttalli, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba coli and Entamoeba polecki ST2). We recorded three Entamoeba species: E. coli (47%), E. dispar (16%), Entamoeba hartmanni (51%). Coproscopically, we could only distinguish the cysts of complex E. histolytica/dispar/moshkovskii/nuttalli and E. coli. Molecular prevalence of entamoebas was higher than the prevalence based on the coproscopic examination. Our molecular phylogenies showed that sequences of E. dispar and E. coli from Issa chimpanzees are closely related to sequences from humans and other NHP from GenBank. The results showed that wild chimpanzees harbour Entamoeba species similar to those occurring in humans; however, no pathogenic species were detected. Molecular-phylogenetic methods are critical to improve diagnostics of entamoebas in wild NHP and for determining an accurate prevalence of Entamoeba species. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
Number of the records: 1