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Nuclear myosin I regulates cell membrane tension

SYSNO ASEP	0467987
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Nuclear myosin I regulates cell membrane tension
Author(s)	Venit, Tomáš (UMG-J)_{RID, ORCID} Kalendová, Alžběta (UMG-J) Petr, Martin (UMG-J) Dzijak, Rastislav (UMG-J)_RID Pastorek, Lukáš (UMG-J) Rohožková, Jana (UMG-J)_RID Malohlava, M. (CZ) Hozák, Pavel (UMG-J)_{RID, ORCID}
Number of authors	8
Article number	30864
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 6, AUG 2 (2016)
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	neuronal growth cone ; rna-polymerase-ii ; cancer cells ; phosphatidylinositol 4,5-bisphosphate ; myo1c ; actin ; transcription ; complex ; motor ; afm
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP305/11/2232 GA ČR - Czech Science Foundation (CSF)
	LO1304 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMG-J - RVO:68378050
UT WOS	000380638000001
DOI	10.1038/srep30864
Annotation	Plasma membrane tension is an important feature that determines the cell shape and influences processes such as cell motility, spreading, endocytosis and exocytosis. Unconventional class 1 myosins are potent regulators of plasma membrane tension because they physically link the plasma membrane with adjacent cytoskeleton. We identified nuclear myosin 1 (NM1) a putative nuclear isoform of myosin 1c (Myo1c) as a new player in the field. Although having specific nuclear functions, NM1 localizes predominantly to the plasma membrane. Deletion of NM1 causes more than a 50% increase in the elasticity of the plasma membrane around the actin cytoskeleton as measured by atomic force microscopy. This higher elasticity of NM1 knock-out cells leads to 25% higher resistance to short-term hypotonic environment and rapid cell swelling. In contrast, overexpression of NM1 in wild type cells leads to an additional 30% reduction of their survival. We have shown that NM1 has a direct functional role in the cytoplasm as a dynamic linker between the cell membrane and the underlying cytoskeleton, regulating the degree of effective plasma membrane tension.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2017

Number of the records: 1