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Dimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC
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SYSNO ASEP 0467891 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC Author(s) Cheel, José (MBU-M) RID, ORCID
Bogdanová, K. (CZ)
Ignatova, S. (GB)
Garrard, I. (GB)
Hewitson, P. (GB)
Kolář, M. (CZ)
Kopecký, Jiří (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Vacek, J. (CZ)Source Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 18, SEP (2016), s. 244-249Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords Phenolic cyclopentenedione ; Natural antibiotic ; Cyanobacteria Subject RIV EE - Microbiology, Virology R&D Projects EE2.3.30.0059 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000381748800028 EID SCOPUS 84976428074 DOI 10.1016/j.algal.2016.06.022 Annotation The need for new antimicrobial agents is greater than ever because of the emergence of multidrug resistance in common pathogens and incidence of new infections. Cyclopent-4-ene-1,3-diones (CPDs) have been reported as a new class of compounds with promising antimicrobial and antifungal properties. Herein we report the selective antibiotic properties of nostotrebin 6, a phenolic CPD produced biotechnologically by the culture of cyanobacterium Nostoc sp. str. Lukesova 27/97. High performance countercurrent chromatography (HPCCC) combined with gel permeation chromatography (GPC) was used for the isolation of nostotrebin 6 with a relatively high 0.53 +/- 0.1% yield (calculated from dried biomass) and final purity higher than 96%. Nostotrebin 6 was tested for its antimicrobial and antifungal activities by using standard micro-dilution method, and the results were expressed as minimal inhibitory concentrations (MICs). Nostotrebin 6 unequivocally inhibited the growth of Gram-positive reference (Enterococcus faecalis CCM 4224, Staphylococcus aureus CCM 4223 and Staphylococcus aureus CCM 3953) and multidrug-resistant (Staphylococcus haemolyticus A/16568, Staphylococcus aureus MRSA 4591 and Enterococcus faecium VanA 419/ana) strains. Its strongest effect was exerted against the Gram-positive bacteria with MICs ranging between 6.25 and 15.6 mu g/mL. There was no effect on Gram-negative strains tested and yeasts. Our results suggest that nostotrebin 6 could serve as basic nucleus for further design of novel antibiotic agents and demonstrate that the bio-production approach based on HPCCC/GPC isolation endpoint is an efficient methodology for obtaining nostotrebin 6 in multi-gram scale. Furthermore, the presented isolation method can be easily up-scaled to process kilograms of the cyanobacterial biomass. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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