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Awakening of a Dormant Cyanobacterium from Nitrogen Chlorosis Reveals a Genetically Determined Program
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SYSNO ASEP 0467890 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Awakening of a Dormant Cyanobacterium from Nitrogen Chlorosis Reveals a Genetically Determined Program Author(s) Klotz, A. (DE)
Georg, J. (DE)
Bučinská, Lenka (MBU-M) RID
Watanabe, S. (JP)
Reimann, V. (DE)
Januszewski, W. (DE)
Sobotka, Roman (MBU-M) RID, ORCID
Jendrossek, D. (DE)
Hess, W. R. (DE)
Forchhammer, K. (DE)Source Title Current Biology. - : Cell Press - ISSN 0960-9822
Roč. 26, č. 21 (2016), s. 2862-2872Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords SP PCC 6803 ; SYNECHOCYSTIS STRAIN PCC-6803 ; STARVATION-INDUCED CHLOROSIS Subject RIV EE - Microbiology, Virology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000387835700021 EID SCOPUS 84992109102 DOI 10.1016/j.cub.2016.08.054 Annotation The molecular and physiological mechanisms involved in the transition of microbial cells from a resting state to the active vegetative state are critically relevant for solving problems in fields ranging from microbial ecology to infection microbiology. Cyanobacteria that cannot fix nitrogen are able to survive prolonged periods of nitrogen starvation as chlorotic cells in a dormant state. When provided with a usable nitrogen source, these cells re-green within 48 hr and return to vegetative growth. Here we investigated the resuscitation of chlorotic Synechocystis sp. PCC 6803 cells at the physiological and molecular levels with the aim of understanding the awakening process of a dormant bacterium. Almost immediately upon nitrate addition, the cells initiated a highly organized resuscitation program. In the first phase, they suppressed any residual photosynthetic activity and activated respiration to gain energy from glycogen catabolism. Concomitantly, they restored the entire translational apparatus, ATP synthesis, and nitrate assimilation. After only 12-16 hr, the cells re-activated the synthesis of the photosynthetic apparatus and prepared for metabolic re-wiring toward photosynthesis. When the cells reached full photosynthetic capacity after 48 hr, they resumed cell division and entered the vegetative cell cycle. An analysis of the transcriptional dynamics during the resuscitation process revealed a perfect match to the observed physiological processes, and it suggested that non-coding RNAs play a major regulatory role during the lifestyle switch in awakening cells. This genetically encoded program ensures rapid colonization of habitats in which nitrogen starvation imposes a recurring growth limitation. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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