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Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation
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SYSNO ASEP 0467644 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation Author(s) Číčová, Z. (DE)
Dejung, M. (DE)
Skalický, Tomáš (BC-A)
Eisenhuth, N. (DE)
Hanselmann, S. (DE)
Morriswood, B. (DE)
Figueiredo, L.M. (PT)
Butter, F. (DE)
Janzen, C. J. (DE)Number of authors 9 Article number 35826 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 6, 25 October (2016)Number of pages 15 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords variant surface glycoprotein ; attachment zone filament ; blood stream forms ; life cycle stages ; paraflagellar rod ; stable transformation ; cell morphogenesis ; ortholog groups ; psi blast ; membrane Subject RIV EB - Genetics ; Molecular Biology Institutional support BC-A - RVO:60077344 UT WOS 000386005700001 EID SCOPUS 84992677292 DOI 10.1038/srep35826 Annotation Trypanosomes are masters of adaptation to different host environments during their complex life cycle. Large-scale proteomic approaches provide information on changes at the cellular level, and in a systematic way. However, detailed work on single components is necessary to understand the adaptation mechanisms on a molecular level. Here, we have performed a detailed characterization of a bloodstream form (BSF) stage-specific putative flagellar host adaptation factor Tb927.11.2400, identified previously in a SILAC-based comparative proteome study. Tb927.11.2400 shares 38% amino acid identity with TbFlabarin (Tb927.11.2410), a procyclic form (PCF) stage-specific flagellar BAR domain protein. We named Tb927.11.2400 TbFlabarin-like (TbFlabarinL), and demonstrate that it originates from a gene duplication event, which occurred in the African trypanosomes. TbFlabarinL is not essential for the growth of the parasites under cell culture conditions and it is dispensable for developmental differentiation from BSF to the PCF in vitro. We generated TbFlabarinL-specific antibodies, and showed that it localizes in the flagellum. Co-immunoprecipitation experiments together with a biochemical cell fractionation suggest a dual association of TbFlabarinL with the flagellar membrane and the components of the paraflagellar rod. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
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