Number of the records: 1
Enhanced thermal stability of the thylakoid membranes from spruce. A comparison with selected angiosperms
- 1.
SYSNO ASEP 0467431 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enhanced thermal stability of the thylakoid membranes from spruce. A comparison with selected angiosperms Author(s) Karlický, Václav (UEK-B) ORCID, RID, SAI
Kurasová, Irena (UEK-B)
Ptáčková, B. (CZ)
Večeřová, Kristýna (UEK-B) RID, SAI, ORCID
Urban, Otmar (UEK-B) RID, ORCID, SAI
Špunda, Vladimír (UEK-B) ORCID, SAI, RIDSource Title Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 130, 1-3 (2016), s. 357-371Number of pages 15 s. Language eng - English Country NL - Netherlands Keywords Norway spruce ; Thermal stability ; Circular dichroism ; Photosystem II organization ; Thylakoid membrane Subject RIV ED - Physiology R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA13-28093S GA ČR - Czech Science Foundation (CSF) Research Infrastructure CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Institutional support RVO:67179843 - RVO:67179843 UT WOS 000385248300029 EID SCOPUS 84966289965 DOI 10.1007/s11120-016-0269-3 Annotation Recently, we have found that thermal stability of photosystem II (PSII) photochemistry in spruce needles is higher than in other plants (barley, beech) cultivated under the same temperatures. In this work, temperature dependences of various characteristics of PSII organization were studied in order to obtain complex information on the thermal stability of PSII function and organization in spruce. Temperature dependency of circular dichroism spectra revealed by about 6 A degrees C higher thermal stability of macrodomain organization in spruce thylakoid membranes in comparison with Arabidopsis and barley ones; however, thermal disintegration of light-harvesting complex of PSII did not significantly differ among the species studied. These results thus indicate that thermal stability of PSII macro-organization in spruce thylakoid membranes is enhanced to a similar extent as thermal stability of PSII photochemistry. Clear-native polyacrylamide gel electrophoresis of preheated thylakoids demonstrated that among the separated pigment-protein complexes, only PSII supercomplexes (SCs) revealed considerably higher thermal stability in spruce thylakoids as compared to Arabidopsis and barley ones. Hence we suggest that higher thermal stability of PSII macro-organization of spruce is influenced by the maintenance of PSII SCs in the thylakoid membrane. In addition, we discuss possible effects of different PSII organizations and lipid compositions on the thermal stability of spruce thylakoid membranes. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2017
Number of the records: 1