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Plasma-Activated Tropoelastin Functionalization of Zirconium for Improved Bone Cell Response

SYSNO ASEP	0466481
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Plasma-Activated Tropoelastin Functionalization of Zirconium for Improved Bone Cell Response
Author(s)	Yeo, G. C. (AU) Santos, M. (AU) Kondyurin, A. (AU) Lišková, Jana (FGU-C)_{RID, ORCID} Weiss, A. S. (AU) Bilek, M. M. M. (AU)
Source Title	ACS BIOMATERIALS SCIENCE & ENGINEERING. - : American Chemical Society - ISSN 2373-9878 Roč. 2, č. 4 (2016), s. 662-676
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	bone ; plasma-activated coating ; titanium ; tropoelastin ; zirconium
Subject RIV	EI - Biotechnology ; Bionics
R&D Projects	NV15-32497A GA MZd - Ministry of Health (MZ)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FGU-C - RVO:67985823
UT WOS	000374083200021
EID SCOPUS	84966266317
DOI	10.1021/acsbiomaterials.6b00049
Annotation	The mechanical strength, durability, corrosion resistance, and biocompatibility of metal alloys based on zirconium (Zr) and titanium (Ti) make them desirable materials for orthopedic implants. However, as bioinert metals, they do not actively promote bone formation and integration. Here we report a plasma coating process for improving integration of such metal implants with local bone tissue. The coating is a stable carbon based plasma polymer layer that increased surface wettability by 28%, improved surface elasticity to the range exhibited by natural bone, and additionally covalently bound the extracellular matrix protein, tropoelastin, in an active conformation. The thus biofunctionalized material was significantly more resistant to medical-grade sterilization by steam, autoclaving or gamma-ray irradiation, retaining >60% of the adhered tropoelastin molecules and preserving full bioactivity. The interface of the coating and metal was robust so to resist delamination during surgical insertion and in vivo deployment, and the plasma process employed was utilized to also coat the complex 3D geometries typical of orthopedic implants. Osteoblast-like osteosarcoma cells cultured on the biofunctionalized Zr surface exhibited a significant 30% increase in adhesion and up to 70% improvement in proliferation. Cells on these materials also showed significant early stage up-regulation of bone marker expression (alkaline phosphatase, 1.8 fold; osteocalcin, 1.4 fold), and sustained up-regulation of these genes (alkaline phosphatase, 1.3 fold; osteocalcin, 1.2 fold) in osteogenic conditions. In addition, alkaline phosphatase production significantly increased (2-fold) on the functionalized surfaces, whereas bone mineral deposition increased by 30% above background levels compared to bare Zr. These findings have the potential to be readily translated to the development of improved Zr and Ti-based implants for accelerated bone repair.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2017

Number of the records: 1