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Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots
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SYSNO ASEP 0465910 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparative study on toxicity of extracellularly biosynthesized and laboratory synthesized CdTe quantum dots Author(s) Komínková, M. (CZ)
Milosavljevic, V. (CZ)
Vítek, Petr (UEK-B) RID, SAI
Polanská, H. (CZ)
Číhalová, K. (CZ)
Dostálová, S. (CZ)
Hynstová, V. (CZ)
Guran, R. (CZ)
Kopel, P. (CZ)
Richtera, L. (CZ)
Masarik, M. (CZ)
Brtnický, M. (CZ)
Kynický, J. (CZ)
Zítka, O. (CZ)
Adam, V. (CZ)Source Title Journal of Biotechnology. - : Elsevier - ISSN 0168-1656
Roč. 241, JAN (2017), s. 193-200Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Quantum dots ; Biosynthesis ; Escherichia coli (E. coli) ; CdTe ; Toxicity Subject RIV EI - Biotechnology ; Bionics OECD category Environmental biotechnology R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Institutional support RVO:67179843 - RVO:67179843 UT WOS 000392677900026 EID SCOPUS 85006584917 DOI 10.1016/j.jbiotec.2016.10.024 Annotation Nanobiosynthesis belongs to the most recent methods for synthesis of nanoparticles. This type of synthesis
provides many advantages including the uniformity in particle shape and size. The biosynthesis
has also a significant advantage regarding chemical properties of the obtained particles. In this study, we
characterized the basic properties and composition of quantum dots (QDs), obtained by the extracellular
biosynthesis by Escherichia coli. Furthermore, the toxicity of the biosynthesized QDs was compared to
QDs prepared by microwave synthesis. The obtained results revealed the presence of cyan CdTe QDs after
removal of substantial amounts of organic compounds, which stabilized the nanoparticle surface. QDs
toxicity was evaluated using three cell lines Human Foreskin Fibroblast (HFF), Human Prostate Cancer
cells (PC-3) and Breast Cancer cells (MCF-7) and the MTT assay. The test revealed differences in the toxicity
between variants of QDs, varying about 10% in the HFF and 30% in the MCF-7 cell lines. The toxicity of
the biosynthesized QDs to the PC-3 cell lines was about 35% lower in comparison with the QDs prepared
by microwave synthesis.Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2018
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