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The method of surface modification for the purpose of enrichment of phosphorylated peptides for analysis by desorption/ionization mass spectrometry techniques

SYSNO ASEP	0465769
Document Type	P - Patent
R&D Document Type	Patent or other outcome protected by special legislation
Title	The method of surface modification for the purpose of enrichment of phosphorylated peptides for analysis by desorption/ionization mass spectrometry techniques
Author(s)	Volný, Michael (MBU-M)_ORCID Novák, Petr (MBU-M)_{RID, ORCID} Havlíček, Vladimír (MBU-M)_{RID, ORCID} Pompach, Petr (MBU-M)_{RID, ORCID}
Year of issue	2015
Possible third party use of the result	A - Pro využití výsledku jiným subjektem je vždy nutné nabytí licence
Royalty requested	A - Poskytovatel licence požaduje licenční poplatek
Patent no. or utility model no. or industrial design no.	EP2652769
Date of the patent acceptance	25.11.2015
Name of the patent owner	Mikrobiologický ústav AV ČR, v. v. i.
Code of the issuer name	EPO_1 - European Patent Office Munich, The Hague, Berlin, Vienna, Brusselshttp://www.epo.org/
Current use	A - Pouze udělený (dosud nevyužívaný) patent nebo patent využívaný jeho vlastníkem
Language	eng - English
Keywords	phosphorelation ; maldi ; FTICR
Subject RIV	EE - Microbiology, Virology
R&D Projects	GPP206/10/P018 GA ČR - Czech Science Foundation (CSF)
	GD204/09/H084 GA ČR - Czech Science Foundation (CSF)
	ME10013 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP206/12/1150 GA ČR - Czech Science Foundation (CSF)
	LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
Annotation	The present invention relates to a method of making modification of solid substrates for efficient surface preconcentration of phosphorylated peptides from complex peptide mixtures prior detection based on desorption-ionization mass spectrometry. The subject of the protection is a method of modifying surfaces used as substrates for desorption-ionization mass spectrometry. The method according to the present invention is based on electronebulization (electrospraying) of a solution of organometallic compounds of the group 4B elements, and namely zirconium, hafnium and titanium. The charged elecrospray thus obtained is dried in real time by passage through a heated space and impinges onto a surface to which it fixedly binds in the form of oxide.; The surface modified in such a manner can then be used for preconcentration of phosphorylated peptides from peptide mixtures with subsequent analysis using desorption-ionization mass spectrometry.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017
Electronic address	https://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20151125&DB=&locale=en_EP&CC=EP&NR=2652769B1&KC=B1&ND=4

Number of the records: 1