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Plant Cell Division: Methods and Protocols
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SYSNO ASEP 0465368 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Synchronization of Green Algae by Light and Dark Regimes for Cell Cycle and Cell Division Studies Author(s) Hlavová, Monika (MBU-M)
Vítová, Milada (MBU-M) RID, ORCID
Bišová, Kateřina (MBU-M) RIDSource Title Plant Cell Division: Methods and Protocols. - Totowa : HUMANA PRESS, 2016 / Caillaud Marie-Cécile - ISBN 978-1-4939-3141-5 Pages s. 3-16 Number of pages 14 s. Number of pages 243 Publication form Print - P Language eng - English Country US - United States Keywords Green algae ; Multiple fission cell cycle ; Synchronization Subject RIV EE - Microbiology, Virology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000369085800002 EID SCOPUS 84949907697 DOI 10.1007/978-1-4939-3142-2_1 Annotation A synchronous population of cells is one of the prerequisites for studying cell cycle processes such as DNA replication, nuclear and cellular division. Green algae dividing by multiple fission represent a unique single cell system enabling the preparation of highly synchronous cultures by application of a light-dark regime similar to what they experience in nature. This chapter provides detailed protocols for synchronization of different algal species by alternating light-dark cycles; all critical points are discussed extensively. Moreover, detailed information on basic analysis of cell cycle progression in such cultures is presented, including analyses of nuclear, cellular, and chloroplast divisions. Modifications of basic protocols that enable changes in cell cycle progression are also suggested so that nuclear or chloroplast divisions can be followed separately. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
Number of the records: 1