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Processing a Biological Tissue from Cryo-SEM to Replica

SYSNO ASEP	0465204
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Processing a Biological Tissue from Cryo-SEM to Replica
Author(s)	Vaškovicová, Naděžda (UPT-D)_{RID, ORCID, SAI} Hrubanová, Kamila (UPT-D)_{RID, SAI, ORCID} Krzyžánek, Vladislav (UPT-D)_{RID, ORCID, SAI}
Number of authors	3
Source Title	Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276 Roč. 22, S3 (2016), s. 236-237
Number of pages	2 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	Cryo-SEM ; ACE600
Subject RIV	JA - Electronics ; Optoelectronics, Electrical Engineering
R&D Projects	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
DOI	10.1017/S1431927616002038
Annotation	Cryo-SEM is a technique suitable for observation of frozen biological samples. The cryo-preparation of tissue fragments without using cryo-protectants can destroy intracellular compartments and cytoplasm. On the other hand the use of cryoprotectants can prevent deeper etching of the medium and thus it often leads to more complicated detection of cell membranes, which are important for orientation in the sample during its observation. This can be managed by shadowing of the fractured structures by platinum and it may help to heighten the structures as is done in production of replicas which can give more detail at higher magnification. Here we report how the replicas can be prepared in the device ACE600 (Leica microsystems) equipped with the cryo-attachement and two guns for metal sputtering and carbon evaporation, and the combination of cryo-SEM and replicas.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2017

Number of the records: 1