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Study of Plant Waxes Using Low Temperature Method for ESEM
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SYSNO ASEP 0465203 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Study of Plant Waxes Using Low Temperature Method for ESEM Author(s) Neděla, Vilém (UPT-D) RID, ORCID, SAI
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Schiebertová, P. (CZ)
Zajícová, I. (CZ)
Schwarzerová, K. (CZ)Number of authors 5 Source Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 22, S3 (2016), s. 1180-1181Number of pages 2 s. Publication form Print - P Language eng - English Country US - United States Keywords ESEM ; plant waxes ; low temperature method Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects GA14-22777S GA ČR - Czech Science Foundation (CSF) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 DOI 10.1017/S1431927616006747 Annotation A possibility to study biological samples in their native state, or even living organisms, using an environmental scanning electron microscope (ESEM) is of great importance for biologists. Sputter-free, electrically non-conductive and wet biological samples in their living and native state are observed using ESEM under conditions of high pressure water vapors that ranges from units to thousands of Pa in the ESEM specimen chamber. One of unwanted consequences of the electron beam-gas interaction is the electrons scattering that results in beam spot defocusing followed by the decrease of the detected signalto-noise ratio. This can be compensated by the reduction of beam electrons gas path length and scanning speed or by the increasing of the beam energy. The latter two parameters fundamentally enhance radiation damage of sensitive and soft native samples and represent a limiting factor for their observation using ESEM. This problem can be overcome by the use of special methods like Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiencies. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2017
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