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Plant Cytogenetics
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SYSNO ASEP 0464630 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Flow Sorting Plant Chromosomes Author(s) Vrána, Jan (UEB-Q) RID, ORCID
Cápal, Petr (UEB-Q) RID, ORCID
Čihalíková, Jarmila (UEB-Q) RID
Kubaláková, Marie (UEB-Q) RID
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Plant Cytogenetics, 1429. - New York : Humana Press, 2016 / Kianian S.F. ; Kianian P.M.A. - ISSN 1064-3745 - ISBN 978-1-4939-3620-5 Pages s. 119-134 Number of pages 16 s. Number of copy 3400 Number of pages 198 Publication form Print - P Language eng - English Country US - United States Keywords Cell cycle synchronization ; Chromosome isolation ; Cytogenetic stocks Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000399777900012 EID SCOPUS 84990226376 DOI 10.1007/978-1-4939-3622-9_10 Annotation Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
Number of the records: 1